Main Page
Deanship
The Dean
Dean's Word
Curriculum Vitae
Contact the Dean
Vision and Mission
Organizational Structure
Vice- Deanship
Vice- Dean
KAU Graduate Studies
Research Services & Courses
Research Services Unit
Important Research for Society
Deanship's Services
FAQs
Research
Staff Directory
Files
Favorite Websites
Deanship Access Map
Graduate Studies Awards
Deanship's Staff
Staff Directory
Files
Researches
Contact us
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Deanship of Graduate Studies
Document Details
Document Type
:
Thesis
Document Title
:
Intracellular Signaling of N-Formyl-Methionyl-Leucyl-Phenylalanine in Human Peripheral Blood Neutrophils
الإشارات الخلوية الداخلية للإن فورمايل ميثونايل ليوسايل فينايل ألانين في خلايا دم الإنسان المتعادلة الأصباغ
Subject
:
Biochemistry department
Document Language
:
Arabic
Abstract
:
Human polymorphonuclear neutrophils (PMN) play a critical role in host defences against invading microorganisms, and, in response to a variety of stimuli. They are a major source of reactive oxygen species (ROS) which are essential for bacterial killing and may induce oxidative stress in tissues. A precise regulation of the oxidase activity is therefore necessary. The respiratory burst is one of the marked responses of neutrophils for stimulation of their chemokine receptors by a chemotactic peptide, such as N-formylmethionylleucylphenylalanine (fMLP). The intracellular signals arising from the fMLP receptors have been proposed to be mediated by heterotrimeric G-protein and phosphoinositide 3-kinase (PI3K), eventually leading to NADPH oxidase activation. fMLP-induced respiratory burst is is accompanied by PI3K activation. In the present study, pharmacologic inhibition of PI-3Kγ activity blocks neutrophil respiratory burst activity stimulated by fMLP. PI3Kγ was shown to play an important role in signal transduction in fMLP-induced respiratory burst in human neutrophils. The aim of this study was to determine the effect of a selective PI3Kγ inhibitor (PI3Kγ inhibitor II) on the superoxide anion production induced by fMLP in human peripheral blood neutrophils collected from healthy donors. The respiratory assay was used to find out the optimal concentration of fMLP with the superoxide dismutase (SOD) as an inhibitor to the reduction of ferricytochrome C with PI3Kγ. By using the PI3Kγ inhibitor II to find out if fMLP activates the release of superoxide anion from neutrophils via a PI3Kγ pathway, or partially dependent on PI3Kγ with another pathway. The results showed that the optimal concentration of fMLP that induced the greatest superoxide production was 100 nM and the optimal concentration of PI3Kɣ that inhibits superoxide anion was 1 nM. It is recommended that additional experiments be conducted using more blood samples and different concentrations of fMLP.
Supervisor
:
Dr. Mohamed Farouk Elshal
Thesis Type
:
Master Thesis
Publishing Year
:
1434 AH
2013 AD
Co-Supervisor
:
Dr. Sawsan Hassan Mahassni
Added Date
:
Tuesday, June 18, 2013
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
حنان حامد العويضي
Al-Owaidhy, Hanan Hamid
Researcher
Master
Files
File Name
Type
Description
35655.pdf
pdf
Back To Researches Page