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Document Type |
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Thesis |
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Document Title |
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ISOLATION AND MOLECULAR PROFILING OF MICROORGANISMS RESPONSIBLE FOR DEGRADING HYDROCARBONS FROM OIL SPILLS ON SAUDI ARABIAN ENVIRONMENT العزل والتوصيف الجزيئي لبعض الميكروبات المسؤولة عن تكسير الهيدروكربونات الناتجه من تسرب النفط فى البيئه السعوديه |
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Subject |
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Faculty of Sciences > biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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Polyaromatic hydrocarbons main component of crude Oil, is one of important pollutant present in environment. Our study aims was to isolate and identify microorganisms (Fungi and bacteria) from crude-oil contaminated environments, which has the ability to degrade petroleum hydrocarbons. About 123 crude-oil-degrading bacteria were isolated from oil-contaminated sites near the Red sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 12 strains were selected from the 123 isolated strains for further study. These 12 strains were selected on the basis of DCPIP Assay. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genus Pseudomonas, Nitratireductor, Bacillus, Staphylococcus, Erwinia and Enterobacter. Among the 12 isolates, strains S5 (Pseudomonas sp., 95 %), 4b (Nitratireductor sp, 70 %) and (Pseudomonas stutzeri, 77 %) were the most effective in degrading crude oil. Rate of crude-oil and Enzyme Assay were observed using spectrophotometer and GC-MS after two week of cultivation in Bushnell Haas medium. Rate of degradation was also increases up to 15 %, after immobilization of Pseudomonas sp. with polyvinyl alcohol. In another study about 27 oil degrading fungi were isolated also from the same oil contaminated sites near Red sea. Three fungal isolates were selected among 27 oil degrading strains, based on their ability to degrade the crude oil and DCPIP assay. The next generation sequencing studies of fungal diversity, ITS1 and ITS2 shows that these strains belongs to genus Aspergillus and Penicillium. Among these three strains Y2 Aspergillus Oryzae (99%) was the most effective crude oil degrading Fungi. The degradation and enzyme activity were observed using spectrophotometer and GC-MS after two weeks of cultivation in BHS medium. Screening for Group II alkane hydrolase also indicate that Y2 express AlkB gene in the presence of crude oil and has almost 98 % sequence similarity with bacterial AlkB gene retrieved from NCBI. Which show that these strains has the ability to degrade and may be use for cleaning the Saudi Arabian environment. |
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Supervisor |
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DR. |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1437 AH
2016 AD |
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Added Date |
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Wednesday, June 1, 2016 |
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Researchers
| ياسر أنور أنور الحق | ANWAR, YASIR ANWAR | Researcher | Doctorate | |
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