Main Page
Deanship
The Dean
Dean's Word
Curriculum Vitae
Contact the Dean
Vision and Mission
Organizational Structure
Vice- Deanship
Vice- Dean
KAU Graduate Studies
Research Services & Courses
Research Services Unit
Important Research for Society
Deanship's Services
FAQs
Research
Staff Directory
Files
Favorite Websites
Deanship Access Map
Graduate Studies Awards
Deanship's Staff
Staff Directory
Files
Researches
Contact us
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Deanship of Graduate Studies
Document Details
Document Type
:
Thesis
Document Title
:
Diversity of HBB haplotypes Among Hemoglobinopathies Patients
مدى تنوع وتفاوت الهابلوتايب لجين البيتا جلوبين في مرضى خضاب الدم (الهيموغلوبين)
Subject
:
Faculty of Applied Medical Sciences
Document Language
:
Arabic
Abstract
:
Hemoglobinopathies are commonly found worldwide and characterized by a wide spectrum of clinical severity and complications. The wide spectrum of clinical symptoms of these disorders in some regions may be caused by the diversity of β-globin gene (HBB) haplotypes which reflect racial and ethnic origin of the affected individuals. This study aimed to investigate HBB haplotype among hemoglobinopathies patients in Jeddah City. 143 samples were recruited from King Abdulaziz Universty Hospital (KAUH), 77 samples analyzed as β-thalassemia, 40 samples analyzed as sickle cell anemia (SCA) and 26 samples as control to normal persons. Determination of HBB haplotypes were performed using polymerase chain reaction amplification of fragments containing four polymorphic restriction sites (PCR-RFLP) around and within HBB. The digested fragments were visualized with ethidium bromide staining after electrophoresis. The study show that haplotype 1 and Benin haplotype are the most prevalent haplotypes with 38% (54 samples) and 33% (48 samples), respectively. The Benin haplotype comprises 80% )32 samples ) of the SCA patients while 52% ( 41samples ) of β-thalassemia samples were found to be haplotype1. Mean of hemoglobin (Hb) concentration was the the highest in Benin haplotype (8.4 g/dl) while the least in Haplotype 3 (5.3g/dl). In other hand, our results showed a strong association between HBB haplotypes and SCA group P=0.000002 while there was no significant with β- thalassemia group P=0.375. Our study show that the Benin haplotype and haplotypes 1 are the highest percentages were found in hemoglobinopathy patients. Additionally it suggests that identified haplotype could be originated from African populations due to close African contient from Western Region of Saudi Arabia or may be was connected together millions years ago. Future study will be aimed to determine of Hb F in each haplotype as well as identify the type of mutation which repeats more in each haplotype in β-thalassemia patients.
Supervisor
:
Dr. Raed Flemban
Thesis Type
:
Master Thesis
Publishing Year
:
1440 AH
2019 AD
Added Date
:
Wednesday, July 31, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
علي دخيل الله دخيل الله السميري
Alsamiri, Ali Dakhil Allah
Researcher
Master
Files
File Name
Type
Description
44828.pdf
pdf
Back To Researches Page